Chem Biol. 2015 Nov 19;22(11):1512-20. doi: 10.1016/j.chembiol.2015.10.008.

Combining Suppression of Stemness with Lineage-Specific Induction Leads to Conversion of Pluripotent Cells into Functional Neurons.

Halder D¹, Chang GE², De D³, Cheong E², Kim KK⁴, Shin I⁵.

 

Abstract

Sox2 is a key player in the maintenance of pluripotency and stemness, and thus inhibition of its function would abrogate the stemness of pluripotent cells and induce differentiation into several types of cells. Herein we describe a strategy that relies on a combination of Sox2 inhibition with lineage-specific induction to promote efficient and selective differentiation of pluripotent P19 cells into neurons. When P19 cells transduced with Skp protein, an inhibitor of Sox2, are incubated with a neurogenesis inducer, the cells are selectively converted into neurons that generate depolarization-induced sodium currents and action potentials. This finding indicates that the differentiated neurons are electrophysiologically active. Signaling pathway studies lead us to conclude that a combination of Skp with the neurogenesis inducer enhances neurogenesis in P19 cells by activating Wnt and Notch pathways. The present differentiation protocol could be valuable to selectively generate functionally active neurons from pluripotent cells.

Copyright © 2015 Elsevier Ltd. All rights reserved.

 

KEYWORDS:

electrophysiology; neurogenesis; pluripotency; small molecules; stemness

PMID:26590637 [PubMed - in process]

(출처_PubMed)

김경규 교수(의과대학 분자세포생물학교실/삼성융합의과학원 겸직)는 질병과 관련된 생체 고분자들의 삼차구조를 연구하여 이 생체 고분자들의 구조와 기능간의 관계, 그리고 이들 간의 상호작용을 연구하고, 이를 통해 이들 생체고분자의 질병과의 연관성을 분자수준에서 연구하고 있다. 이러한 연구를 위하여 생체고분자의 삼차구조를 원자수준에서 가장 직접적으로 관찰할 수 있는 X-선 결정학을 비롯한 각종 구조생물학적 연구 방법, 그리고 분자생물학, 생화학 및 세포생물학적 연구 방법을 이용하고 있다. 현재 본 실험실에서 관심을 가지고 있는 분야는 protein quality control, bacterial pathogenesis, receptor/ligand interaction, Z-DNA, stem cell transcription factors들이고 이러한 생명현상과 관련된 감염질환, 암, 선천면역 등의 원인 및 치료법에 대한 연구를 진행하고 있다. 최근에는 생체고분자와 무기나노입자의 하이브리드 재료를 질병의 진단 및 열치료에 이용하고 동시에 신규 합성효소로 응용하는 연구도 수행 중에 있다.   

▶ 김경규 교수 프로필

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November 19, 2015

Read more at: http://phys.org/news/2015-11-bacterial-protein-stem-cells-neurons.html#jCp 

 

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[국외] Sciencedaily.com  2015-11-19

Bacterial protein can help convert stem cells into neurons